Exosomal transfer of miR-375-3p from pancreatic β cells to hepatocytes impairs hepatic glycogenesis via Rbpj repression

Figure 1 High-fat diet feeding induces pancreas and liver dysfunction in mice. The mice were fed a chow diet or high-fat diet for 18 weeks. A: Fasting blood glucose levels; B: TUNEL staining for apoptotic cells in the pancreas; C: Quantification of TUNEL-positive cells via ImageJ software; D: Immunofluorescence staining for insulin in the pancreas; E: Insulin mRNA levels in the pancreas; F: Quantification of insulin-positive areas using ImageJ software; G: Western blot analysis of AKT/GSK signaling in the liver; H and I: Densitometric quantification of relative protein levels normalized to β-actin performed using ImageJ; J: Liver glycogen content. The experiments were repeated three times. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. Scale bar: 20 μm. Western blot raw data in Supplementary material.