Nicotinamide mononucleotide protects against diabetic nephropathy via IL-6/Rab5-mediated crosstalk between proximal tubular epithelial cells and podocytes

Figure 7 Nicotinamide mononucleotide protection depends on crosstalk between proximal tubular epithelial cell and podocytes. A and B: Western blot analysis of epithelial-to-mesenchymal transition markers in HK-2 cells that received different treatments and quantification of these results (n = 3); C: IL-6 secretion by HK-2 cells that received different treatments (n = 3); D: Co-immunoprecipitation of active Rab5 in podocytes cultured in different conditioned media and quantification of these results; E and F: Co-immunoprecipitation of nephrin and active Rab5 in podocytes cultured in different conditioned media and quantification of the results (n = 3); G and H: Western blot analysis of cytoplasmic nephrin (C-nephrin) and total nephrin (T-nephrin) from podocytes cultured in different conditioned media and quantification of C-nephrin/T-nephrin ratio (n = 3); I: Representative confocal microscopy images of podocytes cultured in different conditioned media (magnification: × 1000; scale bar: 50 μm; red: Cytoskeleton; blue: Nuclei); J: Quantification of podocyte adhesion after culture in different conditioned media (n = 3); K: Representative electron microscopy images of podocyte spreading after culture in different conditioned media (magnification: × 400; scale bar: 20 μm); L: Quantitative analysis of podocyte spreading after culture in different conditioned media (n = 3). ^aP < 0.05; ^bP < 0.001. HG: High-dose glucose; NMN: Nicotinamide mononucleotide.