Nicotinamide mononucleotide protects against diabetic nephropathy via IL-6/Rab5-mediated crosstalk between proximal tubular epithelial cells and podocytes

Figure 5 IL-6/Rab5 signaling mediates nephrin endocytosis in podocytes by promoting crosstalk between HK-2 cells and podocytes. A and B: Co-immunoprecipitation of active Rab5 (Rab5-GTP) in podocytes cultured in different conditioned media and quantification of these results (n = 3); C and D: Representative co-localization images and quantitative of nephrin (green) and active Rab5 (red) in podocytes grown in different conditioned media (magnification: × 630; scale bar: 20 μm); E: Co-immunoprecipitation of nephrin and active Rab5 in podocytes grown in different conditioned media and quantification of these results (n = 3; F: Western blot analysis of cytoplasmic nephrin (C-nephrin) and total nephrin (T-nephrin) after growth in different conditioned media and quantification of the C-nephrin/T-nephrin ratio (n = 3). ^aP < 0.05; ^bP < 0.001. HG: High-dose glucose; NAb: Neutralizing Ab.