Icariin accelerates bone regeneration by inducing osteogenesis-angiogenesis coupling in rats with type 1 diabetes mellitus

Figure 4 Icariin induced osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells. A: Scratch wound assay to show migration of human umbilical vein endothelial cells (HUVECs) cultured with fresh medium (FM) or conditioned medium (CM) and with or without icariin (ICA) (scale bar: 100 μm); B: Quantification of the scratch wound assays; C: Transwell migration assay of HUVECs cultured with FM or CM and with or without ICA (scale bar: 100 μm); D: Quantification of the transwell migration assays; E: Tube formation assay of HUVECs cultured with FM or CM and with or without ICA (scale bar: 100 μm); F: Quantification of the tube formation assays. Data are mean ± SEM (n = 5). ^bP < 0.01 vs control group; ^dP < 0.01 vs CM + control group. ICA: Icariin; FM: Fresh medium; CM: Conditioned medium; Con: Control.