Dietary Nε-(carboxymethyl) lysine affects cardiac glucose metabolism and myocardial remodeling in mice

Figure 3 Dietary N^ε-(carboxymethyl)lysine increases myocardial fibrosis, hypertrophy and apoptosis in mice. A: Mouse myocardial glycogen Periodic Acid Schiff (PAS) staining, Masson’s trichrome staining, and hematoxylin and eosin staining; Scale 100 μm; B and C: Percentage of PAS-positive and fibrotic areas in the mouse myocardium; D: Relative area of myocardial cells in the myocardium; E-I: Western blotting and its relative level of N^ε-(carboxymethyl)lysine (CML), collagen I, B-cell leukemia/lymphoma 2 (Bcl-2) and Bcl-2-associated X (BAX) in the mouse myocardium; J-L: Atrial natriuretic peptide (ANP), Bax, and Bcl-2 mRNA levels in the mouse myocardium. dCML: Dietary CML. n = 6. ^aP < 0.05, ^bP < 0.01, compared with the control (Ctrl) group.