Basic Study
Copyright ©The Author(s) 2021.
World J Diabetes. Jan 15, 2021; 12(1): 19-46
Published online Jan 15, 2021. doi: 10.4239/wjd.v12.i1.19
Figure 4
Figure 4 Screening siRNAs to silence the expression of tenascin-C protein. A: Protein bands; B: Protein expression of tenascin-C (TNC). Rat mesangial cells (RMCs) were transfected with siRNA-TNC-T1, siRNA-TNC-T2, siRNA-TNC-T3, and siRNA-NC. RMCs were cultured in normal glucose (NG, 5.5 mmol/L glucose) medium for 24 h. aP < 0.05 compared with untransfected RMCs cultured with normal glucose concentrations. The level of the tenascin-C protein was detected using Western blot. The results are presented as the mean ± SD of six independent experiments after normalization to GAPDH levels. TNC: Tenascin-C; NC: Negative control; N: Normal glucose.