Metformin regulates inflammation and fibrosis in diabetic kidney disease through TNC/TLR4/NF-κB/miR-155-5p inflammatory loop

Table 1 Sequences of siRNAs used for transfection

Gene	Sequence
TNC-siRNA	siRNA-TNC-T1 sense	5’-GCA UCU GUG AUG AUG ACU ATT-3’
	Anti-sense	5’-UAG UCA UCA UCA CAG AUG CTT-3’
	siRNA-TNC-T2 sense	5’-GCU GAG AAG GGC AGA CAU ATT-3’
	Anti-sense	5’-UAU GUC UGC CCU UCU CAG CTT-3’
	siRNA-TNC-T3 sense	5’-GCA UUU GUG AGG AUG GUU UTT-3’
	Anti-sense	5’-AAA CCA UCC UCA CAA AUG CTT-3’
	NC-siRNA-T	5’-UUC UCC GAA CGU GUC ACG UTT-3’
	Anti-sense	5’-ACG UGA CAC GUU CGG AGA ATT-3’
TLR4-siRNA	siRNA-TLR4	5’-CGA GCU GGU AAA GAA UUU ATT-3’
	Anti-sense	5’-UAA AUU CUU UAC CAG CUC GTT-3’
	NC-siRNA-TLR4 sense	5’-UUC UCC GAA CGU GUC ACG UTT-3’
	Anti-sense	5’-ACG UGA CAC GUU CGG AGA ATT-3’
miR-155-5p inhibitor	Inhibitor	5’-ACC CCU AUC ACA AUU AGC AUU AA-3’
	Inhibitor-NC	5’-CAG UAC UUU UGU GUA GUA CAA-3’

TNC: Tenascin-C; TLR4: Toll-like receptor-4; NC: Normal control.