Vanadium-dependent activation of glucose transport in adipocytes by catecholamines is not mediated via adrenoceptor stimulation or monoamine oxidase activity

Figure 2 Lack of influence of amine oxidase inhibitors and of 100 µmol/L vanadate on basal and insulin-stimulated hexose uptake in rat adipocytes. Rat fat cells (approximately 18 mg lipid/400 µL) were incubated for 45 min with the indicated concentrations of agents without (control, open columns), or with 1 mmol/L pargyline and 1 mmol/L semicarbazide, respectively (grey columns) or in combination (dark columns); then [³H]-2-deoxyglucose uptake (2-DG) was assayed over a 10-min period. The mean ± SE of 4 separate experiments was determined. There was no significant difference between inhibitors and each respective control. Similarly, no difference was found between corresponding conditions (basal or 100 nmol/L insulin-stimulated 2-DG transport) without (left) and with 100 µmol/L vanadate (right, above orange arrow) by the paired t test. 2-DG: 2-Deoxyglucose.