Screening the RFX6-DNA binding domain for potential genetic variants in patients with type 2 diabetes

Table 2 Primer sequences used in DNA amplification of DNA binding domain sequence of RFX6 gene

Primer	Sequence (5’-3’)	Ta (°C)	Size (bp)
RFX6-3	F: 5- CTT ATG TCT ACT CAT TAC CTC -3	50	306
	R: 5- TCA TGC TAT CTG CCT GAC -3
RFX6-4	F: 5- CAG TTC ATT CAG AGT TCA AC -3	56	216
	R: 5- CTT CAT GCA CAA GAG CAG -3
RFX6-5	F: 5- GTC ATC AGG GTT TGC AGT TC -3	50	258
	R: 5- ATT CAA TAG GTA TCA TGC -3
RFX6-6	F: 5- GTA AGT TGA GAA AGA TGC -3	56	258
	R: 5- CAT GTA TTG CTC AGC TTG -3

Ta indicates annealing temperature.