幽门螺杆菌HspA亚基的表达与纯化

图1 克隆至pETH、pQEH上的Hsp A抗原的基因序列和氨基酸序列.

图2 重组质粒的酶切图. M1: DL2000; 1: pGEXH / XhoI+BamH I ; 2: pQEHS/Nco I+BamHI ; 3: pQEH/Nco I+BamHI; 4: pETH/Nco I+BamHI ; M2: DL15 000.

图3 HspA重组蛋白的电泳分析图. M: Marker, (66 200, 43 000, 31 000, 20 100, 14 400); 1: JM109, 2: HspA-His6(pQEHS), 3: HspA(pQEH), 4: HspA(pGEXH), 5: HspA(pETH), 6: BL21.

图4 HspA 纯化蛋白的电泳分析结果. A: Expression and purification of HspA; B: Expression and purification of HspA-His6. M: Marker(66 200, 43 000, 31 000, 20 100, 14 400); 1: Centrifuged Supernatant; 2: Centrifuged precipitate; 3: loading peak fraction; 4: Equilibrating peak fraction; 5: Eluting peak fraction.

图5 重组蛋白HspA-His6 and HspA的免疫印记分析. M: Marker(97 400, 66 200, 43 000, 31 000, 20 100, 14 400); 1: HspA-His6; 2: HspA.