Case Report
Copyright ©The Author(s) 2022.
World J Clin Cases. Oct 16, 2022; 10(29): 10655-10662
Published online Oct 16, 2022. doi: 10.12998/wjcc.v10.i29.10655
Figure 1
Figure 1 Histopathological changes, Karyotype and polymerase chain reaction and reverse dot blot observed in this case. A: Hematoxylin and eosin staining; 100× bone marrow smear showing platelets in clusters; B: Hematoxylin and eosin staining of the trephine biopsy section (100×, 200×). The ratio of bone marrow hematopoietic tissue to fat cells was 80%:20%, and the granulocyte-to-erythrocyte ratio was approximately 1-2:1; granulocyte hyperplasia (+), erythroid hyperplasia (++), 25-30 megakaryocytes in each high-power field, a local clustering trend, pleomorphism, and reticular fibers (+) were all observed. Pathological diagnosis: right posterior superior spine bone marrow biopsy demonstrated chronic myeloproliferative neoplasms, characterized by megakaryocyte hyperplasia; C: Karyotype showing 46 chromosomes, [A1] including XX chromosomes (20 cells examined); D: PCR-based reverse dot blot showing β-IVS-Ⅱ-654-thalassemia.