P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes

Figure 1 ATP induces phosphatidylserine exposure in erythrocytes from multiple dog breeds. Erythrocytes, from 17 pedigree and eight crossbreed (X) dogs, in NaCl medium were incubated for 24 h at 37 °C in the absence or presence of 1 mmol/L ATP. Cells were labeled with FITC-conjugated Annexin V and analyzed by flow cytometry. The bars represent group means (A and B). A: The symbols represent the percentage of Annexin V⁺ erythrocytes, from each dog, following incubation in the absence (basal) or presence of ATP; ^bP < 0.0001 ATP vs basal; B: The symbols represent the percentage of ATP-induced PS exposure in erythrocytes, from each dog, determined as the difference in the percentage of Annexin V⁺ erythrocytes following incubation in the presence and absence of ATP. The broken line represents the mean ATP-induced PS exposure from all dogs. The symbols for Staffordshire bull terrier X represent a Staffordshire bull terrier and Australian kelpie cross, Staffordshire bull terrier and bull terrier cross, or a Staffordshire bull terrier cross. PS: Phosphatidylserine.