Binge ethanol intake in chronically exposed rat liver decreases LDL-receptor and increases angiotensinogen gene expression

Figure 4 Angiotensionogen (A) and c-fos mRNA (B) expression in chronic and chronic-binge treated rats. After 4 wk of chronic ethanol feeding, single binge was administered as in Figure 1. Total RNA was isolated from liver and reverse transcribed to cDNA. Aliquots of the cDNA preparations were amplified by real time QT-PCR. The fold increase in gene expression was determined after normalizing the differences in level of GAPDH mRNA. Values are mean ± SD (n = 4). ^aP < 0.05 vs control; ^cP < 0.05 vs chronic ethanol group.