Multistep carcinogenesis of perihilar cholangiocarcinoma arising in the intrahepatic large bile ducts

Figure 4 Immunohistochemical expression of p16^INK4a and EZH2 in BilIN-1 and BilIN-2. A: p16^INK4a is intensely and frequently expressed in the cytoplasm and nuclei in BilIN-1 (left), but such expression is unclear in BilIN-2 (right); B: While EZH2 is occasionally expressed in the nuclei in BilIN-1 (left), this expression increases markedly in BilIN-2 (right)[22].