Basic Study
Copyright ©The Author(s) 2019.
World J Stem Cells. Mar 26, 2019; 11(3): 196-211
Published online Mar 26, 2019. doi: 10.4252/wjsc.v11.i3.196
Figure 1
Figure 1 Characterization of isolated nucleus pulposus-derived stem cells. A: Nucleus pulposus-derived stem cells (NPSCs) at P0 and P3 showing a homogeneous fibroblast-like morphology and vortex performance; B: NPSCs at P3 showing colony-like growth ability at a density of 1000 cells/well; C: Multilineage differentiation capacities of NPSCs in vitro. Alizarin Red staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for chondrogenic differentiation; D: Flow cytometric analysis of NPSCs surface markers revealing high expression of the mesenchymal stem cells markers CD73, CD90, and CD105, and low expression of the hematopoietic markers CD34 and CD45. FITC: Fluorescein isothiocyanate; PE: Phycoerythrin.