Copyright ©2009 Baishideng.
World J Stem Cells. Dec 31, 2009; 1(1): 55-66
Published online Dec 31, 2009. doi: 10.4252/wjsc.v1.i1.55
Table 3 Various cell types and the nanotopographies on which they are cultured
Cell typeNanotopographyAdvantagesRef.
Chondrocytes(a) PCL nanofibrous scaffold (200-800 nm) in the presence of TGF-β1; (b) Collagen nanofibers of diameter 110 nm-1.8 μmThe differentiation of the stem cells into chondrocytes in the nanofibrous scaffold was comparable to an established cell pellet culture. Nanotopography supports chondrocyte growth and infiltration[82,90]
Osteoblasts(a) Ceramics like HA, alumina and titania having nanostructures of grain sizes less than 100 nm and nanophase zinc oxide (23 nm); (b) PLGA, PLLA and PCL nanofibers (diameter 200-800 nm); (c) Nanotubes of diameter less than 100 nmEnhanced proliferation and differentiation of MSC to osteoblasts[67,77-79,105-113]
Smooth muscle cells (SMC)(a) PLGA and PCL, PLLA-CL nanofibers (diameter 200-800 nm); (b) Nanogratings of 350 nm in width, spacing, and depth imprinted on PMMA or PDMSSMC adhesion was enhanced on the nanostructured substrates compared to the conventional submicron substrates[114-118]
Fibroblasts(a) PLGA (85:15 ratio) nanofibers of diameter 500-800 nm; (b) NanocolumnsIncreased endocytic activity. Nanotopography can be used to improve hemocompatibility of blood-contacting biomaterials[82]
Nerve cells(a) Silicon wafer in the range of 20-70 nm; (b) PLLA or PCL scaffolds via electrospinning and phase separationThe cell adhesion and viability significantly improved on the nanofeatured surface[70,91]