Copyright
©The Author(s) 2015.
世界华人消化杂志. 2015-10-08; 23(28): 4507-4514
在线出版 2015-10-08. doi: 10.11569/wcjd.v23.i28.4507
在线出版 2015-10-08. doi: 10.11569/wcjd.v23.i28.4507
表1 9种肝螺旋杆菌亚种的16SrRNA
| Strain | RDP No. | 16S rRNA index No. |
| Helicobacter hepaticus; ATCC51449 | S000628343 | AE017125 |
| Helicobacter hepaticus; ATCC51448 | S000390965 | AF302103 |
| Helicobacter hepaticus; FRED1 | S000435927 | L39122 |
| Helicobacter hepaticus; Hh-1 | S000334524 | U07573 |
| Helicobacter hepaticus (T); Hh-2 | S000334525 | U07574 |
| Helicobacter hepaticus; Hh-3 | S000334526 | U07575 |
| Helicobacter hepaticus; MIT 96-284 | S000573920 | AY631952 |
| Helicobacter hepaticus; MIT 96-1809 | S000573919 | AY631950 |
| Helicobacter hepaticus | S000089425 | AJ007931 |
表2 引物序列及反应条件
| Primer | Sequences(5'→3') | Product size(bp) | Reaction condition | ||
| Denaturation | Annealing | Extension | |||
| Outer | ATCAGCCTATGTCCTATCAG | 935 | 94.0 ℃ | 57.0 ℃ | 72.0 ℃ |
| forward | 30 s | 30 s | 5 min | ||
| Ouer reverse | CTCACCTTCCTCCTCCTT | ||||
| Inner forward | AGCGTTACTCGGAATCAC | 300 | 94.0 ℃ | 56.0 ℃ | 72.0 ℃ |
| Inner reverse | GACAAGCAAGGCAACAAC | 30 s | 30 s | 3 min | |
引文著录: 秦和平, 毕丽红, 韦碧柳, 潘信义, 叶安丽, 易平. 一种检测肝螺杆菌套式PCR方法的建立及临床应用. 世界华人消化杂志 2015; 23(28): 4507-4514