shRNA干预IGF-ⅠR活化对肝癌细胞增殖的抑制作用

表1 IGF-ⅠR shRNA真核表达质粒及阴性对照质粒序列

名称	正、反义链(5'→3')
IGF-ⅠR-shRNA1	5'-CACCGCTGATGTGTACGTTCCTGATTTCAAGAGAATCAGGAACGTACACATCAGCTTTTTTG-3'
	5'-GATCCAAAAAAGCTGATGTGTACGTTCCTGATTCTCTTGAAATCAGGAACGTACACATCAGC-3'
IGF-ⅠR-shRNA2	5'-CACCGCGTGAGAGGATTGAGTTTCTTTCAAGAGAAGAAACTCAATCCTCTCACGCTTTTTTG-3'
	5'-GATCCAAAAAAGCGTGAGAGGATTGAGTTTCTTCTCTTGAAAGAAACTCAATCCTCTCACGC-3'
IGF-ⅠR-shRNA3	5'-CACCGCAAAGTCTTTGAGAATTTCCTTCAAGAGAGGAAATTCTCAAAGACTTTGCTTTTTTG-3'
	5'-GATCCAAAAAAGCAAAGTCTTTGAGAATTTCCTCTCTTGAAGGAAATTCTCAAAGACTTTGC-3'
IGF-ⅠR-shRNA4	5'-CACCGGACAGATCCTGTGTTCTTCTTTCAAGAGAAGAAGAACACAGGATCTGTCCTTTTTTG-3'
	5'-GATCCAAAAAAGGACAGATCCTGTGTTCTTCTTCTCTTGAAAGAAGAACACAGGATCTGTCC-3'
Negative-shRNA	5'-CACCGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTTG-3'
	5'-GATCCAAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAAC-3'

IGF-ⅠR: 胰岛素样生长因子-Ⅰ型受体; shRNA: 短发夹型RNA.

表2 shRNA4与索拉非尼联合抑制Bel-7404(PLC/PRF/5)细胞增殖 (n = 3)

分组(nmol/L)	对照组		Neg-shRNA		shRNA4 plus Sorafenib		t值		P值
	Bel-7404	PLC/PRF/5	Bel-7404	PLC/PRF/5	Bel-7404	PLC/PRF/5	Bel-7404	PLC/PRF/5	Bel-7404	PLC/PRF/5
0	0.297±0.06	0.373±0.02	0.310±0.07	0.336±0.06	0.199±0.07	0.199±0.07	2.377	-2.39	0.045	-0.044
2.5	0.326±0.10	0.360±0.08	0.335±0.11	0.325±0.10	0.188±0.00	0.158±0.02	3.086	-2.450	0.002	-0.040
5	0.337±0.11	0.351±0.05	0.318±0.05	0.324±0.11	0.191±0.06	0.178±0.05	2.606	-2.447	0.031	-0.040
10	0.331±0.01	0.331±0.10	0.284±0.17	0.284±0.07	0.152±0.09	0.152±0.09	4.42	-1.977	0.002	-0.048
20	0.093±0.03	0.093±0.03	0.054±0.02	0.054±0.02	0.033±0.03	0.033±0.03	4.45	-3.162	0.002	-0.013
40	0.000±0.00	0.003±0.00	0.000±0.00	0.002±0.00	0.003±0.00	0.003±0.00	-	-	-	-

shRNA: 短发夹型RNA.

表3 shRNA4与Oxaliplation联合抑制Bel-7404(PLC/PRF/5)细胞增殖 (n = 3)

分组(nmol/L)	对照组		Neg-shRNA		shRNA4 plus Sorafenib		t值		P值
	PLC/PRF/5	Bel-7404	PLC/PRF/5	Bel-7404	PLC/PRF/5	Bel-7404	PLC/PRF/5	Bel-7404	PLC/PRF/5	Bel-7404
0.0	2.391±0.30	2.929±0.08	2.351±0.17	2.662±0.29	1.429±0.07b	2.291±0.11	6.983	10.489	<0.001	<0.001
5.0	1.064±0.21	2.006±0.29	1.014±0.05	1.879±0.11	0.677±0.08b	1.636±0.30	3.851	1.983	0.005	0.083
10.0	0.659±0.16	1.670±0.25	0.575±0.03	1.431±0.12	0.417±0.13a	1.034± 0.18b	2.625	4.617	0.030	0.002
20.0	0.288±0.12	1.178±0.13	0.280±0.05	1.179±0.15	0.168±0.02	0.655±0.09b	2.206	7.396	0.059	<0.001
40.0	0.164±0.04	0.247±0.03	0.162±0.01	0.319±0.06	0.099±0.04a	0.145±0.03b	2.569	5.376	0.033	0.001

^aP<0.05,

^bP<0.01 vs 对照组.