E2F-1基因过表达对胃癌细胞动力学影响机制

表1 MTT法测各组各时间点A值 (mean±SD)

分组	24 h	48 h	72 h	96 h	120 h	144 h	168 h
MGC-803/E2F-1	0.011±0.001a	0.020±0.002a	0.029±0.001a	0.095±0.004a	0.158±0.005a	0.187±0.003a	0.334±0.006a
MGC-803/EV	0.025±0.002	0.068±0.003	0.107±0.006	0.310±0.006	0.755±0.013	0.875±0.015	1.075±0.024
MGC-803	0.031±0.001	0.063±0.001	0.112±0.004	0.334±0.007	0.827±0.009	0.846±0.016	1.331±0.029

^aP<0.05 vs MGC-803组.

表2 各组细胞增殖率比较 (mean±SD)

分组	增值率(%)	95%CI
MGC-803/E2F-1	26.61±5.19a	[21.80, 31.41]
MGC-803/EV	93.40±10.29	[83.94, 102.97]
MGC-803	100.00±0.00	[100.00, 100.00]

^aP<0.05 vs MGC-803组.

表3 各组细胞的细胞周期分布比较 (mean±SD, %)

分组	G0/G1期	S期	G2/M期
MGC-803/E2F-1	70.63±2.45a	16.90±2.77a	12.47±3.74
MGC-803/EV	60.03±3.42	28.70±1.77	11.27±4.54
MGC-803	60.43±2.02	29.70±1.50	10.70±2.29

^aP<0.05 vs MGC-803组.

表4 与细胞生长、增殖和细胞周期有关的差异表达基因

GenBank	Gene symbol	Pathway	Ratio
NM_000546	TP53	cell growth	2.0404
		cell cycle
NM_000820	GAS6	cell growth	2.0778
		cell proliferation
NM_001321	CSRP2	cell growth	2.5802
		cell proliferation
NM_018361	PLCE1	cell growth	0.4372
NM_014011	SOCS5	cell growth	0.2701
NM_015934	NOP5/NOP58	cell growth	0.3928
NM_006373	VAT1	cell growth	2.6922
NM_022157	RRAGC	cell growth	2.6514
NM_020244	CHPT1	cell growth	0.3288
NM_002087	GRN	cell proliferation	4.0888
NM_004494	HDGF	cell proliferation	0.4607
NM_002467	MYC	cell proliferation	0.1875
NM_002032, NR_002205	FTH1	cell proliferation	2.6732
NM_016082, NM_016408	CDK5RAP1	cell proliferation	0.3539
NM_032637, NM_005983	SKP2	cell cycle	0.4021
NM_006904	PRKDC	cell cycle	2.1083
NM_006190	ORC2L	cell cycle	0.1933
NM_002358	MAD2L1	cell cycle	0.3756
NM_006306	SMC1L1	cell cycle	0.5192
NM_078467, NM_000389	CDKN1A	cell cycle	4.3546