临床研究
Copyright ©The Author(s) 2009.
世界华人消化杂志. 2009-04-08; 17(10): 985-991
在线出版 2009-04-08. doi: 10.11569/wcjd.v17.i10.985
表1 9个基因的引物序列
引物序列5'→3'片段大小(bp)退火温度( ℃)
G3PDHCTC ATG ACC ACA GTC CAT GCC ATC ACT G14055
CAT GAG GTC CAC CAC CCT GTT GCT GTA
GPC3TGA CCT GAA AGT ATT TGG GAA TTT CC42760
CAA TAG TGG TGG TCA GCT TTC CTG
E2F1GAA CCA CAT CCA GTG GCT GG52063
GGA CAA CAG CGG TTC TTG CT
MMP2GAA GGC TGT GTT CTT TGC AGG32561
TCA GGT AAT AGG CAC CCT TGA AG
CLDN10TGT CAG GGC TGT GCT CAA TG15057
CCA CCA ATT ATG CAC AGT GAG G
HGFGAA AAC AAT GCC TCT GGT TCC20057
TGT GTT CGT GTG GTA TCA TGG A
PTENTAG AGT TCT TCC ACA AAC AGA ACA GA55060
AAT CTG ACA CAA TGT CCT ATT GCC
DLC1TTT ACC ACC TGC TGA ATT ACT CAC GCC30060
ACG TCT GTG TAG TGC ACA GGA AAG CC
PRDM2GGG ATG GAT GTG CAT TGA TG35061
GCA GAG GTG AAA TCT GGC TCA
MGMTGTG CAC AGC CTG GCT GAA T50062
CGC ATC CGA TGC AGT GTT AC
表2 基因表达量和分子诊断指数
GPC3E2F1MMP2CLDN10HGFPTENDLC1PRDM2MGMTIndex
NL11.2681.2540.5750.9471.3790.6000.7370.7521.6520
NL21.5281.2711.4061.4061.2781.2681.8030.8690.3220
NL31.1271.8231.0080.8600.6301.6381.9321.5671.4080
NL40.3470.5610.8360.5561.1130.6290.7020.4960.9820
NL51.3210.6141.4661.5710.8091.2760.5551.9701.3600
HV2192.5701.7242.8132.5182.1801.1340.9660.7520.7980
HV2291.0810.6100.5081.0302.4020.9282.3781.0201.4480
HV2380.8192.5420.8361.6843.9560.6941.0351.2380.7291
HV2390.8481.0110.1542.0450.9890.0921.1491.2290.7812
LC2240.4940.8680.3691.8052.2410.6382.3130.4821.1360
LC2324.3231.9001.2411.0583.4441.3682.9281.6222.5392(1)
LC2410.3721.0540.2470.4902.7210.5442.5490.8221.2871
LC2431.7931.0040.1621.6961.9640.3770.5630.5030.5521
LC2473.4870.9500.4212.7932.3690.6560.5140.7011.0241
LC2486.5072.9000.9285.3225.1480.9611.3381.7151.6073(1)
LC2504.2344.7441.2853.4632.1801.0081.4440.8460.5803(2)
LC2531.5070.8330.6563.2992.2100.3423.2490.6681.0102(1)
LC2543.3090.4190.0371.3491.5410.1140.6200.3320.3473
LC2550.1940.3940.0270.5011.0380.0670.5320.1330.4164
LC2560.7080.3370.2590.3747.1800.0801.3471.2550.6393(2)
LC2597.5798.4331.0732.1325.3292.2381.6250.9450.9423(2)
120N20.4270.1760.1810.4771.2350.3851.8280.3790.5762
121N20.9031.5540.6160.5522.6830.2861.7650.8460.4161
122N20.4670.1920.6291.0963.7950.4121.9320.8171.0032(1)
123N21.9211.4011.2505.1772.0910.5482.4451.3550.7091(0)
124N21.9892.9200.9341.0301.8581.1111.4041.1711.1200
125N22.5530.3780.7540.6791.8330.4302.9691.4120.8850
127N218.7911.3620.1833.8422.9570.0911.2920.6680.7874(3)
128N20.4180.3360.1620.3410.8150.4010.5590.1840.5602
130N20.3540.1620.4900.7641.7950.2470.9730.3760.5012
131N21.0730.7000.1820.5370.9690.4011.6700.1791.0902
132N21.2330.3430.2450.5671.0240.3340.9460.4000.6661
133N252.7835.5251.3973.7374.2990.3344.4381.8761.0535(3)
134N22.81312.7820.1690.7230.7760.0160.3900.5130.1854
137N20.4420.3940.4152.1611.6180.0940.7530.5060.4551
143N211.0962.2131.0739.6602.6100.0972.0991.7031.5413(2)
145N20.3850.0640.1100.5751.6070.3420.6290.4790.3672
146N20.0930.0270.0790.3740.4020.0690.2570.1040.1947
120T190.2831.5010.0540.0390.0170.0390.6550.3580.0366
121T107.0405.1550.3960.0700.3310.2090.3100.1810.0917
122T220.09810.3102.6980.3041.2431.3681.8662.7660.6133
123T3.3452.5781.3120.0040.8980.5951.9860.9991.0672
124T306.9804.4880.3260.0060.1710.5672.8480.2660.8676
125T0.0421.4910.3150.0340.0740.0190.1040.4320.0216
127T14.1432.1670.2111.7311.3140.0120.7020.4790.3224
128T127.2925.2632.1610.2630.5120.3870.5030.6020.3304
130T234.2658.5500.2130.0070.0730.2960.4630.2040.5887
131T35.5556.0040.2080.4670.3000.3821.1890.1010.3575
132T333.6068.3740.5260.9030.6570.4871.5580.5130.3233
133T576.8298.7910.2290.0540.3260.0120.5511.5350.1557
134T1.57118.0760.1610.1460.0970.0340.1110.0560.0758
137T71.60612.5190.4770.0030.7711.9752.1140.3250.6904
143T3.7360.6722.9324.8301.0530.0100.5400.7260.1974
145T326.7402.8400.2160.2190.1490.5220.9590.1410.3206
146T29.0811.5540.0880.0030.1860.3870.2770.0880.1767
表3 9个基因在各组中的相对表达 (mean±SD)
组织类型nGPC3E2F1MMP2CLDN10HGFPTENDLC1PRDM2MGMT
正常51.12±0.45a1.10±0.52a1.06±0.381.07±0.411.04±0.32c1.08±0.45ae1.15±0.661.13±0.611.14±0.52ae
肝炎41.33±0.841.47±0.85a1.08±1.191.81±0.632.38±1.22a0.71±0.451.38±0.671.06±0.230.94±0.34a
肝硬化122.87±2.46a1.99±2.40a0.56±0.462.02±1.48a3.11±1.83a0.70±0.631.59±0.970.83±0.491.00±0.62ae
癌旁(2 cm)125.33±12.99a1.67±3.18a0.50±0.401.53±1.55a1.82±1.08ace0.34±0.251.49±1.060.69±0.500.63±0.32
17151.84±163.515.90±4.710.74±0.940.54±1.190.48±1.43c0.43±0.520.98±0.810.57±0.680.37±0.30
P0.0000.0030.3590.0450.0000.0240.4070.2440.000

引文著录: 王伟丽, 高英堂, 杜智, 王毅军, 景丽, 郭华, 杨斌, 白同. 应用实时荧光定量RT-PCR法建立肝癌分子诊断指数. 世界华人消化杂志 2009; 17(10): 985-991