肝癌转移抑制基因在8p21.1-23.1染色体上的功能定位

表1 人类染色体8p21.1-23.1上的STS

STS	在染色体上位置(bp)	寡核甘酸序列	产物长度(bp)
D8S2099	9988829-9989037	F: GGGATGATGAAACGTGGG
		R: TTCAGCTCTTTGACTATGTTATCCC	209
D8S453	10681928-10682191	F: GGTAGGCAACAAGTATTCTGGTTG
		R: TGGTGATAAAGCAAGACTCTGTCT	264
D8S265	11317148-11317364	F: ACCTCTTTCCAGATAAGCCC
		R: CCAATGGTTTCGGTTACTGT	208-231
D8S552	12752557-12752676	F: CCTGTACCATACCCCTGTATC
		R: AAGGTTTGAATCTCTCAGTGG	132
D8S1754	13000035-13000211	F: CAGGGAAGTCTCGGTTTG
		R: TCAGGGACACGATTCAGC	169-183
D8S17271	13600230-13600446	F: CCAGAAAACTTTCACCACCGC
		R: GATGAGCCTTGGAAACACGCT	217
SHGC-110274	14903937-14904209	F: GATTCGATCTCCTTACTCTTTATTGG
		R: CGATAAGCAAAAATCCACAGACC	273
D8S484	16075640-16076081	F: ACCGCAACTCGAACAAAACAAAAAC
		R: ATGTGAAAAGGCCTAGTATTCAGAC	442
D8S2081	17097196-17097342	F: ACCCAGTTACAGCACTGTAATATCA
		R: CTCTACCCCGAAATGATGGA	147
D8S1611	17732205-17732310	F: ATTCTGAACATATCCATGCGC
		R: GCATATCTGCTGTACTGAAAGCA	105-106
D8S2001	19262474-19262611	F: GACATTGAATTCCAGTATTTGTGC
		R: GGACAAATGCCACTGCAAC	138
D8S1660	19736326-19736527	F: TCCTAGATACCGTCCAAACCC
		R: GTGGATAAGGACAGGAAAAGAGG	200-201
D8S282	21425116-21425356	F: TCCTATTCCACAGCCACAACTC
		R: CTTATATTGGGCATAAAGTTCATAGG	237
D8S439	22271321-22271581	F: GGCTCCCTGTTCTTTATCAGGTTG
		R: ACTTTTCTCTGGCTATTATGGACTC	261-351
D8S1734	22851217-22851336	F: GCTATCCACTTGTCCCAGA
		R: AGCCCAGAAATAAACCCTC	98-120
D8S2028	23445977-23446155	F: CAAAAGTTTTGTTTCTATTCAGGG
		R: TTTTTTCTGTTCCCCTCCG	178
D8S441	23860052-23860227	F: TGTCTTCTCCAGTGAGCTCCAGT
		R: GAGATGATAGATAGCGGTGTGTGT	176
D8S1839	27404471-27404651	F: AGAATGGGATCAGAAC
		R: TCCAGGGTTGCTACAGT	161-193
D8S2133	28938060-28938204	F: CATTTCTTTGTAGGTATTTTGTGGG
		R: AGTTTCATGAGAATGCTGAAGTAGG	145
D8S1996	29760749-29761003	F: ACTGGAAAAATGAAGTGTAAAAACC
		R: CTCCAGTTCCTGTGGCTTTT	254-255
D8S1769	31203002-31203253	F: ATGGGGGTTATGGGACTACA
		R: CGTAACAGGCAACATAAGTAGAGTG	240-256
D8S254	16652480-16652550	F: TGCCGGACATACATTAGTGA
		R: TTGTAAACACCACAAGCAGG	65-75
D8S1591	18465968-18466176	F: CAAGATTTCTTTTTATTCACCTGC
		R: TTTCTTTAGATGGAGTCCATTGC	208-209
D8S1733	22576582-22576836	F: CACAGTCTCAAACTCCTGGG
		R: ACGAAAAACCATGAACAAGA	217-259
D8S1589	26335774-26335953	F: TGAGACACTGTCGATTTATTTTAGC
		R: TCCTGTTTTTCCTGTACCGG	179-180
D8S1973	28445735-28445988	F: AGTGTTTATTAACAAATGGGCTCA
		R: TCAACTCTGGCTCTCTCAAGG	253-254

表2 人类染色体8p21.1-23.1区段上STS位点的筛选

STS	A9	C5F	转移不抑制克隆		转移抑制克隆
			A9/C5F-1	A9/C5F-2	A9/C5F-4	A9/C5F-8	A9/C5F-10
DD8S2099	+	-	+	+	+	+	+
D8S453	+	-	+	+	+	+	+
D8S265	+	-	+	+	+	+	+
D8S552	+	-	-	-	-	-	+
D8S1754	+	-	+	+	+	+	+
D8S17271	+	-	+	+	+	+	+
SHGC-110274	+	-	+	+	+	+	+
D8S484	+	-	+	+	+	+	+
D8S254	+	-	-	-	-	-	+
D8S2081	+	-	+	+	+	+	+
D8S1611	+	-	+	+	+	+	+
D8S1591	+	-	+	-	+	-	+
D8S2001	+	-	+	+	+	+	+
D8S1660	+	-	+	+	+	+	-
D8S282	+	-	+	+	+	+	+
D8S439	+	-	+	+	+	+	+
D8S1733	+	-	-	-	-	+	+
D8S1734	+	-	-	-	+	-	-
D8S2028	+	-	+	+	+	+	+
D8S441	+	-	+	+	+	+	+
D8S1589	+	-	-	+	+	+	+
D8S1973	+	-	-	-	-	-	+
D8S1839	+	-	+	+	+	+	+
D8S2133	+	-	+	+	+	+	+
D8S1996	+	-	+	+	+	+	+
D8S1769	+	-	+	+	+	+	+