外周血单个核细胞TRAIL mRNA和血清sTRAIL水平与HBV感染肝损伤的相关性

表1 引物和探针序列

引物和探针		序列	位置
TRAIL	S	5'-CCCAATGACGAAGAGAGTATGAAC-3'	270nt
	AS	5'-TGTAGAAATGGTTTCCTCAGAGGT-3'	377nt
	Probe	5'-FAM-CTGGCAAGTCAAGTGGCAACTCCGT-TAMRA-3'	302nt
β2M	S	5'-TCCATCCGACATTGAAGTTGAC-3'	213nt
	AS	5'-ACTATCTTGGGCTGTGACAAAG-3'	398nt
	Probe	5'-FAM-TGGTTCACACGGCAGGCATACTCA-TAMRA-3'	371nt

表2 各型肝病患者PBMCs中TRAIL mRNA水平、血清sTRAIL水平与肝功指标相关分析(mean±SD)

分组	n		血清sTRAIL(ng/L)	PBMCs TRAIL mRNA	ALB(g/L)	TBIL (μmol/L)	ALT(nkat/L)
慢性肝炎	轻度	13	571.5±314.9	0.785±0.036b	43.4±3.9	15.4±5.0	1478.6±1477.0
	中度	16	533.4±139.9a	0.765±0.032b	41.3±4.3	20.3±11.8	4525.9±5957.9
	重度-重型	7	496.9±106.4b	0.761±0.049b	38.0±4.9	71.2±69.3	16751.7±9083.5
肝硬化		22	436.8±130.2b	0.780±0.041b	33.1±5.8	43.4±54.3	1138.6±833.5

^aP<0.05,

^bP<0.001 vs 健康对照组.

表3 血清sTRAIL水平、PBMCs中TRAIL mRNA水平与血清HBV DNA浓度相关分析(mean±SD)

血清HBV DNA(copies/L)	n	PBMCs TRAIL mRNA	血清sTRAIL(ng/L)
阴性	14	0.786±0.039	508.8±173.6
4×(106-108)	10	0.781±0.023	466.8±110.2
108-109	21	0.764±0.037	499.0±285.8
>109	13	0.762±0.025	514.8±167.9