结肠癌耐药细胞株LoVo/5-FU的建立及其差异表达基因的筛选

表1 LoVo/5-FU细胞的耐药谱

耐药倍数(RI) = 耐药细胞IC50/亲本细胞IC50
5-FU	7.69
ADM	2.78
MMC	1.43
CDDP	0.96

表2 经验证的基因芯片筛选出的相关基因

基因序号	基因名称	Cy5/Cy3*
3855c02	Homo sapiens cytochrome P450, family 1, subfamily B, polypeptide 1. (CYP1B1)	5.877
3854a04	Homo sapiens N-acetyltransferase 2 (arylamine N-acetyltransferase, NAT2)	5.755
1256h07	Homo sapiens ring finger protein 20 (RNF20)	2.762
3599e01	Homo sapiens snail homolog 2 (Drosophila) (SNAI2)	2.272
1553a08	Homo sapiens mitogen-activated protein kinase kinase kinase 2 (MAP3K2)	2.126

Cy5(实验组), Cy3(对照组), Cy3信号值乘上均一化系数得出调整后的Cy3*, 计算每个基因点在本次实验中的表达差异值Ratio, Ratio = Cy5/Cy3*.