5-烷基间苯二酚诱导人结直肠癌细胞凋亡的机制探讨

图1 显微镜下不同浓度5ARs干预HT29 24 h后细胞的形态变化. A: HT29-对照组; B: HT29-5ARs(5 μmol/L)组; C: HT29-5ARs(10 μmol/L)组; D: HT29-5ARs(15 μmol/L)组; E: HT29-5ARs(20 μmol/L)组. 5ARs: 5-烷基间苯二酚.

图2 显微镜下不同浓度5ARs干预HCT116 24 h后细胞的形态变化. A: HCT116-对照组; B: HCT116-5ARs(5 μmol/L)组; C: HCT116-5ARs(10 μmol/L)组; D: HCT116-5ARs(15 μmol/L)组; E: HCT116-5ARs(20 μmol/L)组. 5ARs: 5-烷基间苯二酚.

图3 CCK8法检测不同浓度5ARs对HT29、HCT116增殖能力的调控(24 h). A: 0: 空白对照组; 1: 阴性对照组; 2: HT29-5ARs(5 μmol/L)组; 3: HT29-5ARs(10 μmol/L)组; 4: HT29-5ARs(15 μmol/L)组; 5: HT29-5ARs(20 μmol/L组; B: 0: 空白对照组; 1: 阴性对照组; 2: HCT116-5ARs(5 μmol/L)组; 3: HCT116-5ARs(10 μmol/L)组; 4: HCT116-5ARs(15 μmol/L)组; 5: HCT116-5ARs(20 μmol/L)组. ^bP<0.01 vs 1组. 5ARs: 5-烷基间苯二酚.

图4 Annexin V-FITC/PI流式细胞术检测5ARs诱导人HT29及HCT116凋亡. A: 1: 阴性对照组; 2: HT29-5ARs(5 μmol/L)组; 3: HT29-5ARs(10 μmol/L)组; 4: HT29-5ARs(15 μmol/L)组; 5: HT29-5ARs(20 μmol/L)组; B: 1: 阴性对照组; 2: HCT116-5ARs(5 μmol/L)组; 3: HCT116-5ARs(10 μmol/L)组; 4: HCT116-5ARs(15 μmol/L)组; 5: HCT116-5ARs(20 μmol/L)组. ^bP<0.01 vs 1组. 5ARs: 5-烷基间苯二酚.

图5 Western-blot检测5ARs对HT29中PARP1、Caspase3、Bax及BCL2的表达的影响(A, B). ^aP<0.05, ^bP<0.01 vs 1组. 1: 阴性对照组; 2: HT29-5ARs(5 μmol/L)组; 3: HT29-5ARs(10 μmol/L)组; 4: HT29-5ARs组; 5: HT29-5ARs组. 5ARs: 5-烷基间苯二酚.

图6 Western-blot检测5ARs对HT29中Bax/BCL2比值的影响. ^aP<0.05, ^bP<0.01 vs 1组. 1: 阴性对照组; 2: HT29-5ARs(5 μmol/L)组; 3: HT29-5ARs(10 μmol/L)组; 4: HT29-5ARs组; 5: HT29-5ARs组. 5ARs: 5-烷基间苯二酚.

图7 Western-blot检测5ARs对HCT116中PARP1、Caspase3、MCC1、A2F及BCL2的表达的影响(A, B). ^aP<0.05, ^bP<0.01 vs 1组. 1: 阴性对照组; 2: HCT116-5ARs(5 μmol/L)组; 3: HCT116-5ARs(10 μmol/L)组; 4: HCT116-5ARs(15 μmol/L)组; 5: HCT116-5ARs(20 μmol/L)组. 5ARs: 5-烷基间苯二酚.

图8 Western-blot检测5ARs对HCT116中Bax/BCL2比值的影响. ^bP<0.01 vs 1组. 1: 阴性对照组; 2: HCT116-5ARs(5 μmol/L)组; 3: HCT116-5ARs(10 μmol/L)组; 4: HCT116-5ARs(15 μmol/L)组; 5: HCT116-5ARs(20 μmol/L)组.