基因沉默血管内皮生长因子受体3对胃癌细胞增殖的抑制作用

图1 pSUPER-siRNA/VEGFR3质粒的酶切鉴定. 1: 1000 bp Marker; 2: pSUPER-siRNA1/VEGFR3 HindⅢ和EcoRⅠ双酶切后出现284 bp的条带为阳性克隆; 3: pSUPER空载体双酶切后出现241 bp的条带为阴性克隆; 4: pSUPER-siRNA2/VEGFR3 HindⅢ和EcoRⅠ双酶切后出现284 bp的条带为阳性克隆; 5: pSUPER-siRNA3/VEGFR3 HindⅢ和EcoRⅠ双酶切后出现284 bp的条带为阳性克隆; 6: 空质粒pSUPER未经酶切; 7: pSUPER经HindⅢ单酶切; 8: pSUPER-siRNA2/VEGFR3 BglⅡ单酶切.

图2 VEGFR3 mRNA的表达. 1: LoVo; 2: LoVo-siRNA1/VEGFR3; 3: LoVo-siRNA2/VEGFR3; 4: LoVo-siRNA3/VEGFR3; 5: LoVo-pSUPER; ^cP<0.05.

图3 VEGFR3 siRNA的蛋白表达. 1: pSUPER-siRNA1/VEGFR3; 2: pSUPER-siRNA3/VEGFR3; 3: pSUPER空质粒; 4: pSUPER-siRNA2/VEGFR3.

图4 SGC7901细胞生长曲线. ^cP<0.05 vs 对照组.