基础研究
Copyright ©The Author(s) 2006.
世界华人消化杂志. 2006-09-18; 14(26): 2605-2609
在线出版 2006-09-18. doi: 10.11569/wcjd.v14.i26.2605
图1
图1 琼脂糖凝胶电泳分析PCR扩增的目的基因. 1: DNA Marker; 2: Lpp20; 3: HspA; 4: CagA; 5: UreaseA; 6: UreaseB.
图2
图2 重组表达质粒的双酶切鉴定. 1: Lpp20/pGEX-4T-1 (EcoRⅠ和SalⅠ); 2 HspA/pGEX-4T-1(BamHⅠ和EcoRⅠ); 3: CagA/pGEX-4T-1(BamHⅠand SalⅠ); 4 UreasA/pGEX-4T-1(BamHⅠ和EcoRⅠ); 5: UreaseB/pGEX-4T-1(EcoRⅠ和SalⅠ); 6 DNA Marker.
图3
图3 SDS-PAGE分析目的基因在大肠杆菌中的表达产物. 1: Lpp20/pGEX-4T-1; 2: HspA/pGEX-4T-1; 3: UreaseA/pGEX-4T-1; 4: CagA/pGEX-4T-1; 5: UreaseB/pGEX-4T-1; 6: GEX-4T-1; 7: protein Marker.
图4
图4 SDS-PAGE分析纯化后的蛋白. 1: Lpp20; 2: HspA; 3: UreaseA; 4: CagA; 5: UreaseB; 6: protein Marker.
图5
图5 免疫印迹分析. 1: Lpp20; 2: HspA; 3: UreaseA; 4: CagA; 5: UreaseB; 6: health serum; 7: protein Marker.
引文著录: 宁云山, 李妍, 龙敏, 董文其, 李明. 幽门螺杆菌5种候选疫苗抗原基因的克隆、表达及抗原性的鉴定. 世界华人消化杂志 2006; 14(26): 2605-2609