内皮抑素-可溶性血管内皮细胞生长抑制因子融合基因重组腺病毒的包装与鉴定

图1 IL3/hENDO-linker和sVEGI基因PCR扩增电泳图. 1: λDNA/ EocRⅠ+HindⅢ marker(21227 bp, 5148 bp, 4269 bp, 3530 bp, 2027 bp, 1904 bp, 1587 bp, 1375 bp, 941 bp, 831 bp, 564 bp); 2: 647 bp的IL3/hENDO-linker片段; 3: 476 bp的VEGI片段.

图2 pCA13 -hENDO-sVEGI质粒的构建流程示意图. 1: λDNA/ EcoRⅠ+HindⅢ marker(21 227 bp, 5 148 bp, 4 269 bp, 3 530 bp, 2 027 bp, 1 904 bp, 1 587 bp, 1 375 bp, 941 bp, 831 bp, 564 bp); 2: Digesting with HindⅢ and BamHⅠ(1 114 bp, 6.95 kb); 3: Digesting with HindⅢ and EcoRⅠ (647 bp, 7.49 kb); 4: Digesting with EcoRⅠand BamHⅠ(476 bp, 7.69 kb).

图3 pCA13 -hENDO-sVEGI质粒的目的基因酶切鉴定. 1: λDNA/ EcoRⅠ+HindⅢ marker (21227 bp, 5148 bp, 4269 bp, 3530 bp, 2027 bp, 1904 bp, 1587 bp, 1375 bp, 941 bp, 831 bp, 564 bp); 2: Digesting with HindⅢ and BamHⅠ(1114 bp, 6.95 kb); 3: Digesting with BglⅡ(1707 bp, 6.3 kb); 4: Digesting with SacⅠ(1535 bp, 2.0 kb, 4.5 kb).

图4 pCA13 -hENDO-sVEGI质粒目的基因插入部位的酶切鉴定.