Original Articles
Copyright ©The Author(s) 1999.
World J Gastroenterol. Dec 15, 1999; 5(6): 465-469
Published online Dec 15, 1999. doi: 10.3748/wjg.v5.i6.465
Figure 2
Figure 2 Targeted luciferase gene expression. Conjugates containing 1μg of CMV luc were added to Huh7 (ASG receptor positive) or SK Hep1 cells (ASG receptor negative) and incubated for 48 h as describ ed in Materials and Methods. Gene expression was measured by luciferase detection using the luciferin substrate, and detection of activity using a luminometer. Luciferase expression results were standardized by measuring protein concentrati ons according to the Bradford assay. Panel A, Huh7 cells: ASOR-PL-DNA, lane 1; ASOR-PL-LLO-DNA, lane 2; ASOR-PL-LLO-DNA+200-fold molar excess of ASOR, lane 3; ASOR-PL-DNA+LLO, lane 4; DNA alone, lane 5. Panel B, SK Hep1 cells: AS OR-PL-DNA, lane 1; ASOR-PL-LLO-DNA, lane 2; ASOR-PL DNA+LLO, lane 3; DNA a lone, lane 4.