A DNA delivery system containing listeriolysin O results in enhanced hepatocyte-directed gene expression

Figure 2 Targeted luciferase gene expression. Conjugates containing 1μg of CMV luc were added to Huh7 (ASG receptor positive) or SK Hep1 cells (ASG receptor negative) and incubated for 48 h as describ ed in Materials and Methods. Gene expression was measured by luciferase detection using the luciferin substrate, and detection of activity using a luminometer. Luciferase expression results were standardized by measuring protein concentrati ons according to the Bradford assay. Panel A, Huh7 cells: ASOR-PL-DNA, lane 1; ASOR-PL-LLO-DNA, lane 2; ASOR-PL-LLO-DNA+200-fold molar excess of ASOR, lane 3; ASOR-PL-DNA+LLO, lane 4; DNA alone, lane 5. Panel B, SK Hep1 cells: AS OR-PL-DNA, lane 1; ASOR-PL-LLO-DNA, lane 2; ASOR-PL DNA+LLO, lane 3; DNA a lone, lane 4.