MiR-32-5p aggravates intestinal epithelial cell injury in pediatric enteritis induced by Helicobacter pylori

Figure 2 SMAD family member 6 is sponged by miR-32-5p in intestinal epithelial cells. A: Luciferase assay for determining the binding between miR-32-5p and SMAD family member 6 (SMAD6); B: Protein level of SMAD6 in intestinal epithelial cells transfected with miR-32-5p mimic; C: Expression of SMAD6 in intestinal epithelial cells after SMAD6 overexpression and knockdown; D: Cell viability measurement in intestinal epithelial cells after SMAD6 overexpression in the presence or absence of H. pylori; E: Cell viability measurement in intestinal epithelial cells after SMAD6 knockdown in the presence or absence of Helicobacter pylori (H. pylori); F: The mRNA level of TNF-α in H. pylori-infected intestinal epithelial cells after SMAD6 overexpression; H: The mRNA level of TNF-α in H. pylori-infected intestinal epithelial cells after SMAD6 knockdown; G: IL-6 expression in H. pylori-infected intestinal epithelial cells after SMAD6 overexpression; I: IL-6 expression in H. pylori-infected intestinal epithelial cells after SMAD6 knockdown. ^aP < 0.05, ^bP < 0.01. SMAD6: SMAD family member 6; H. pylori: Helicobacter pylori.