Near-infrared photoimmunotherapy of pancreatic cancer using an indocyanine green-labeled anti-tissue factor antibody

Figure 2 Tissue factor expression in human pancreatic cancer cell lines. A: Tissue factor (TF) expression in the cell lines BxPC-3 (lane 1) and SUIT-2 (lane 2) was examined by western blotting; B: Both cell lines were incubated with or without indocyanine green-labeled anti-TF antibody 1849 (1849-ICG) for 18 h and examined under a fluorescent microscope. Intracellular localization of intense fluorescent signals indicated substantial binding and internalization of 1849-ICG within BxPC-3, but very rarely in SUIT-2 cells (Scale bar = 50 μm, 40 × objective magnification). DAPI: 4,6-diamidino-2-phenylindole; 1849-ICG: Indocyanine green-labeled anti-tissue factor antibody 1849; TF: Tissue factor.