Hydrogen-rich water protects against inflammatory bowel disease in mice by inhibiting endoplasmic reticulum stress and promoting heme oxygenase-1 expression

Figure 8 Lack of heme oxygenase-1 reverses the protective role of hydrogen-rich water in dextran sulfate sodium-induced inflammatory bowel disease. Mice were sacrificed, and the colons were rapidly removed and processed for analysis on the 7^th d after inflammatory bowel disease (IBD) modeling. A: The representative pictures of the colon in each group; B: The macroscopic score of the colon in each group; C: The length of the colon in each group; D: The thickness of the colon in each group; E: Hematoxylin-eosin staining of colon tissues (magnification ×100). n = 6, mean ± SEM, ^aP < 0.05 vs dextran sulfate sodium (DSS) group; ^bP < 0.05 vs DSS + HRW group. HO-1: Heme oxygenase-1; HRW: Hydrogen-rich water; HE: Hematoxylin and eosin.