Effect of toll-like receptor 3 agonist poly I:C on intestinal mucosa and epithelial barrier function in mouse models of acute colitis

Table 1 Primers used for real-time quantitative polymerase chain reaction analysis

Gene	Primers		Length, bp
zo-1	Forward	5’-TCATCCCAAATAAGAACAGAGC-3	198
	Reverse	5’-GAAGAACAACCCTTTCATAAGC-3’
Occludin	Forward	5’-CTTTGGCTACGGAGGTGGCTAT-3’	86
	Reverse	5’-CTTTGGCTGCTCTTGGGTCTG-3’
Claudin-1	Forward	5’-GCTGGGTTTCATCCTGGCTTCT-3’	110
	Reverse	5’-CCTGAGCGGTCACGATGTTGTC-3’
IL-17	Forward	5’-TATCCCTCTGTGATCTGGGAAG-3’	161
	Reverse	5’ATCTTCTCGACCCTGAAAGTGA-3’
IFN-γ	Reverse	5’-ATGAACGCTACACACTGCATCTT-3’	139
	Forward	5’-TTTCTTCCACATCTATGCCACTT3’
TNF-α	Reverse	5’-GGTTCTGTCCCTTTCACTCACT-3’	169
	Forward	5’-GAGAAGAGGCTGAGACATAGGC-3’
GAPDH	Reverse	5’-GAGACCTTCAACACCCCAGC-3’	263
	Forward	5’-ATGTCACGCACGATTTCCC-3’

IL-17: Interleukin 17; IFN-γ: Interferon-γ; TNF-α: tumor necrosis factor-α; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.