Improved experimental model of hepatic cystic hydatid disease resembling natural infection route with stable growing dynamics and immune reaction

Figure 7 Pathological images of mouse liver injected with 200 protoscolices at different time points. 0 d: On the same day of portal vein injection, the protoscolices congested the portal vein. 1 d: The injected protoscolice caused the dilatation of vessel sinusoids. The central vein size increased. 3 d: Those dead protoscolices ended up as focal degeneration; mouse liver reacted with increased diameter of central veins, inflammatory cell infiltration (blue stained), and collagen deposition (red stained). 1 wk: Mouse liver had protective immune reactions such as lymphocyte infiltration. The vessel fibrosis was found in biliary ducts and portal vein area. 3 wk: The newly developed hydatid cyst was found full of the germinal layer, the laminated layer, and the beginning of the adventitious layer. 6 wk: The mouse localized the hydatid cyst with compressed and fibrotic host tissue (HE staining, × 10).