Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Jul 7, 2016; 22(25): 5769-5779
Published online Jul 7, 2016. doi: 10.3748/wjg.v22.i25.5769
Figure 4
Figure 4 Urotensin II elevates reactive oxygen species production and NADPH oxidase subunit expression. HepG2 cells were cultured with 0, 1, 10, or 100 nmol/L urotensin II (UII) for 24 h, with or without prior stimulation with apocynin (200 μmol/L, 30 min). Intracellular reactive oxygen species (ROS) production was elevated in a dose- and time-dependent manner (A, B). NADPH oxidase subunits gp91phox, p67phox, p47phox, and p40phox were significantly increased in response to UII (100 nmol/L, 24 h) (C). Apocynin pretreatment inhibited ROS generation (D). Data from at least three independent experiments are presented as the mean ± SD. aP < 0.05, bP < 0.01 vs control group; gP < 0.05 vs UII treatment alone.