Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Nov 21, 2015; 21(43): 12361-12369
Published online Nov 21, 2015. doi: 10.3748/wjg.v21.i43.12361
Figure 2
Figure 2 Status of p-Ser307 insulin receptor substrate-1 and p-Ser473 Akt phosphorylation in hepatitis C virus infected hepatoma cells upon insulin treatment. Total cellular lysates were prepared from hepatitis C virus (HCV) infected and mock infected Huh7.5 cells that were treated or untreated with insulin (100 nmol/L). An equal amount of cellular lysates were subjected to Western blot assay using anti-p-IRS-1 Ser307 and anti-p-Akt Ser473. Cellular actin was used as a protein loading control in each lane. IRS: Insulin receptor substrate.