Evidence-Based Medicine
Copyright ©The Author(s) 2015.
World J Gastroenterol. Apr 28, 2015; 21(16): 5039-5048
Published online Apr 28, 2015. doi: 10.3748/wjg.v21.i16.5039
Figure 2
Figure 2 Real-time polymerase chain reaction melting curve analyses that identified the hepatitis B virus preS1 deletion variants (WT, 15-bp del, 18-bp del, or 21-bp del) with deletions at the start codon of preS1 with cloned positive control plasmids and non-template controls. The Tms were determined using duplicate runs and are presented as average and standard deviation values. The Y-axis is the negative differential of fluorescence over temperature at the detection channel and is normalized using background fluorescence at channel 530. PCR: Polymerase chain reaction; HBV: Hepatitis B virus; WT: Wild type; 15, 18, 21-bp del: 15, 18, 21-base pair deletion; HCC: Hepatocellular carcinoma; LC: Liver cirrhosis; CH: Chronic hepatitis; C: Carrier.