Topic Highlight
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Oct 21, 2014; 20(39): 14292-14300
Published online Oct 21, 2014. doi: 10.3748/wjg.v20.i39.14292
Table 1 Possible methods of sample processing
Sample typesAncillary methods
Direct cytological smearsCytomorphological diagnostic evaluation
ICC staining
Molecular testing (FISH)
Excellent source for isolation of good quality DNA[39,40]
Cell blocks (formalin-fixed, paraffin-embedded tissue)IHC
Various molecular tests
Tissue storage
Touch imprint slides (tumor cell rich slides made by imprinting the tumor)IHC
Molecular testing (FISH)
Fresh aspirated specimens (especially of lymph nodes)Flow cytometry - immunophenotyping
RNA based gene expression studies
DNA based studies[41]
Fresh frozen (cryopreserved) FNA specimensRNA based gene expression studies
DNA based studies[42]
FTA cards (filter paper)Effective RNA and DNA preservation
Not suitable for morphology[43]
Formalin fixed paraffin embedded core tissueHistological morphologic evaluation
IHC and molecular studies