Epidermal growth factor upregulates serotonin transporter and its association with visceral hypersensitivity in irritable bowel syndrome

Figure 5 Role of epidermal growth factor receptor in the regulation of serotonin transporter levels and function in intestinal epithelial cells. IEC-6 cells were pre-treated with an epidermal growth factor receptor (EGFR) inhibitor (10 μmol/L PD153035) prior to stimulation with epidermal growth factor (EGF). A: Serotonin transporter (SERT) was detected by Western blot (^aP < 0.05 vs control, EGF + PD153035, and PD153035) and quantified relative to GAPDH; B: Serotonin (5-HT) reuptake was estimated by an [³H]-5-HT uptake assay. Data are mean ± SD of at least three independent experiments.