MAWBP and MAWD inhibit proliferation and invasion in gastric cancer

Figure 1 Analysis of expression of mitogen-activated protein kinase activator with WD40 repeats binding protein and mitogen-activated protein kinase activator with WD40 repeats in six gastric cancer cell lines. A: mRNA expression for mitogen-activated protein kinase activator with WD40 repeats (MAWD)/MAWD binding protein (MAWBP) in BGC823, MGC803, SGC7901, AGS, N87 and MKN45 six gastric cancer cell lines was detected by reverse transcription-polymerase chain reaction (PCR). There was a low level of endogenous MAWBP and MAWD mRNA in SGC7901 cells, and a high level in BGC823 cells; B: Expression of MAWBP and MAWD proteins was detected by Western blotting. The expression level was lower in SGC7901 cells than in the other cell lines. Expression of MAWBP and MAWD was higher in BGC823 cells than in the other cells; C: mRNA expression for MAWBP and MAWD was detected by real-time PCR. There were lower levels of endogenous MAWD and MAWBP mRNA in SGC7901 cells, and higher levels in BGC823 cells. NTC: No template control.