Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells

Figure 2 Double immunostaining was used to analyze the cellular localization of high-mobility group box 1 protein and α-smooth muscle actin in hepatic fibrosis tissue (original magnification, × 200). A: α-smooth muscle actin (α-SMA) was stained with polyclonal α-SMA antibody and secondarily by rhodamine -conjugated anti-rabbit antibody (green); B: High-mobility group box 1 (HMGB1) was stained with monoclonal anti-HMGB1 antibody and secondarily by fluoresceinisothiocyanate-conjugated anti-rabbit antibody (red); C: The yellow areas on the merged image show co-localization of α-SMA and HMGB1.