(-)-Epigallocatechin-3-gallate inhibits VEGF expression induced by IL-6 via Stat3 in gastric cancer

Figure 2 (-)-Epigallocatechin-3-gallate inhibits interleukin-6-induced vascular endothelial growth factor expression in human gastric cancer cells via JAK/STAT pathway. Human gastric cancer (AGS) cells were stimulated with interleukin-6 (IL-6) (50 ng/mL) for 24 h in the presence of 50 μmol (-)-epigallocatechin-3-gallate (EGCG) or signaling inhibitors. Vascular endothelial growth factor (VEGF) protein levels in tumor cell lysates were analyzed by Western blotting. IL-6 markedly increased VEGF expression in AGS cells. When treated with EGCG or AG490, VEGF expression was significantly reduced. PD98059 and LY294002 did not change IL-6-induced VEGF expression. 1: Without IL-6 stimulation; 2: Stimulated with IL-6; 3-6: Treated with 50 μmol EGCG or signaling inhibitors of 20 μmol AG490, 25 μmol PD98059 or 25 μmol LY294002.