Original Article
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Dec 21, 2009; 15(47): 5924-5935
Published online Dec 21, 2009. doi: 10.3748/wjg.15.5924
Figure 2
Figure 2 TRAIL-induced apoptosis in hepatocellular carcinoma (HCC) cells. Huh7 and Hep-G2 cells were seeded onto 96-well plates and treated on day one after seeding with different TRAIL compounds. A: Cells were treated for 48 h with rec. TRAIL + 1 μg/mL Enhancer as indicated; B: Plates were coated with crosslinker IgG F(ab)’2 for 24 h before seeding of cells. Cells were then treated for 48 h with LBY135 + 1 μg/mL F(ab)’2 as indicated; C: Cells were treated for 48 h with SkTRAIL as indicated. Cell viability was analyzed by MTT assay. Viability is shown relative to untreated controls. Assays were performed in six-fold values and are representative of three independent experiments. Values are expressed as mean ± SD. Enh: Enhancer.