Brief Article
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Nov 28, 2009; 15(44): 5549-5557
Published online Nov 28, 2009. doi: 10.3748/wjg.15.5549
Figure 5
Figure 5 SCFAs inhibit LPS-induced production of TNF-α and IFN-γ in human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner. Human PBMC isolated by Ficoll-Hypaque centrifugation procedure (1 × 106 cells/mL) were incubated with the indicated concentrations of short-chain fatty acids (SCFA) or 100 nmol/L dexamethasone (Dex), and then cultured for 22 h with or without 100 ng/mL LPS. Culture supernatants were analyzed for 8 proinflammatory cytokines including IL-1β, IL-2, IL-6, IL-8, IL-10, IL-12p70, IFN-γ, TNF-α by Meso Scale Multi-Spot Discovery Technology. The data for TNF-α (A) and IFN-γ (B) with LPS stimulation are shown here.