Iron increases HMOX1 and decreases hepatitis C viral expression in HCV-expressing cells

Figure 3 Effects of FeNTA on Bach1 protein levels in Huh-7, 9-13, and CNS3 cells. A: Bach1 protein levels in Huh-7 cells; B: Bach1 protein levels in 9-13 cells; C: Bach1 protein levels in CNS3 cells. Huh-7, 9-13 or CNS3 cells were treated with different concentrations of FeNTA (0, 50, 100 μmol/L) or 100 μmol/L NaNTA for 16 h, after which cells were harvested and total protein was isolated, as described in Material and Methods. Proteins were separated on 4%-15% SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with anti-human Bach1 and GAPDH specific antibodies. The relative amounts of Bach1 were normalized to GAPDH.