Brief Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Sep 28, 2009; 15(36): 4499-4510
Published online Sep 28, 2009. doi: 10.3748/wjg.15.4499
Figure 2
Figure 2 Effects of FeNTA on Nrf2 protein levels in Huh-7, 9-13, and CNS3 cells. A: Nrf2 protein levels in Huh-7 cells; B: Nrf2 protein levels in 9-13 cells; C: Nrf2 protein levels in CNS3 cells. aP < 0.05 vs control. Huh-7, 9-13 or CNS3 cells were treated with different concentrations of FeNTA (0, 20, 50, 100 μmol/L) or 100 μmol/L NaNTA for 6 h, after which cells were harvested and total protein was isolated, as described in Materials and Methods. Proteins were separated on 4%-15% SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with anti-human Nrf2 and GAPDH specific antibodies. The relative amounts of Nrf2 proteins were normalized to GAPDH.