Basic Research
Copyright ©2007 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 14, 2007; 13(46): 6183-6190
Published online Dec 14, 2007. doi: 10.3748/wjg.v13.i46.6183
Figure 5
Figure 5 Effects of CORM-2 on NF-κB activation in the ileum tissue of thermally injured mice. Mice were challenged with thermal injury and treated with CORM-2 as described in Figure 1. Measurement of NF-κB activity was performed by EMSA with 32P-labeled NF-κB probe and 5 μg nuclear extract from the ileum of sham, burn, burn + DMSO and burn+CORM-2 mice at 24 h after thermal injury. NF-κB activation in the ileum of thermally injured mice was markedly increased (lane 2), and this activity was inhibited by CORM-2 (lane 4). A representative experiment is shown in A, and quantitative results (average optical density) of three experiments are shown in B. aP < 0.05 vs sham-treated; cP < 0.05 vs burned.