Basic Research
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Jun 7, 2006; 12(21): 3359-3367
Published online Jun 7, 2006. doi: 10.3748/wjg.v12.i21.3359
Figure 2
Figure 2 Effect of solanine on the morphology of HepG2. A: Control group, PMT1 nuclear chromatin was stained green and showed normal structure, while PMT2 showed no or only weak red fluorescence; B: Group treated with Solanine with final concentration of 0.0032 μg/mL: The cells show slightly pyknotic and crumb-shaped structures. Permeability of cell membrane has increased, so that AO and EB can enter the cells, resulting in the cells showing both green and red fluorescence; C: Group treated with Solanine with final concentration of 0.016 μg/mL: The cells show slightly pyknotic and crumb-shaped structure. Fragments or apoptotic bodies appear in the nuclei of some of the cells; D: Group treated with solanine with final concentration of 0.08 μg/mL: Cell structure is further damaged. The cells are not only stained with AO and EB, but morphologically the nuclei have become deeply stained fragments or apoptotic bodies; E: Group treated with solanine with final concentration of 0.4 μg/mL: Cell structure is damaged even further. Apoptotic bodies have definitely appeared; F: Group treated with solanine with final concentration of 2 μg/mL: Definite apoptotic bodies can be seen, while the number of cells in sight has decreased; G: Group treated with camptothecin with final concentration of 2 μg/mL: The appearance of apoptotic bodies is obvious in HepG2 cells.

Citation: Gao SY, Wang QJ, Ji YB. Effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells. World J Gastroenterol 2006; 12(21): 3359-3367