Effects of protein deprivation and re-feeding on P2X2 receptors in enteric neurons

Figure 1 Co-localization of P2X₂ receptor immunoreactivity with nitric oxide synthase, calbindin, calretinin and choline acetyltransferase immunoreactivity in the ileal myenteric plexus in the N, D and DR groups. A-C: P2X₂ receptor-IR (green) co-localized with nitric oxide synthase (NOS)-IR (red); D-F: P2X₂ receptor-IR (green) co-localized with calbindin-IR (red); G-I: P2X₂ receptors (green) co-localized with calretinin (red); J-L: P2X₂ receptors (green) co-localized with choline acetyltransferase (ChAT) (red). Double-labeled neurons are indicated by arrows.

Figure 2 Co-localization of P2X₂ receptor immunoreactivity with calbindin, calretinin and choline acetyltransferase immunoreactivity in the ileal submucosal plexus in N, D and DR groups. A-C: P2X₂ receptor (green) co-localized with calbindin (Calb) (red); D-F: P2X₂ receptor (green) co-localized with calretinin (Calr) (red); G-I: P2X₂ receptor (green) co-localized with choline acetyltransferase (ChAT) (red). Double-labeled neurons are indicated by arrows.

Figure 3 The density (neuron/cm²) of neurons immunoreactive for the P2X₂ receptor, nitric oxide synthase, calbindin, calretinin, and choline acetyltransferase in the ileal myenteric plexus in the N, D and DR groups. ^aP < 0.02, ^bP < 0.01, ^cP < 0.002, ^dP < 0.001 vs N and DR groups. NOS: Nitric oxide synthase; ChAT: Choline acetyltransferase.

Figure 4 Calculated numbers of neurons immunoreactive for the P2X₂ receptor, nitric oxide synthase, calbindin, calretinin and choline acetyltransferase in the small intestine of the N, D and DR groups. ^aP < 0.05 vs N and DR groups. NOS: Nitric oxide synthase; ChAT: Choline acetyltransferase.

Figure 5 The density of neurons (neuron/cm²) immunoreactive for P2X₂ receptor, calbindin, calretinin and choline acetyltransferase in the ileal submucosal plexus in the N, D and DR groups. ^aP < 0.01, ^bP < 0.001, ^cP < 0.0003 vs N and DR groups. ChAT: Choline acetyltransferase.

Figure 6 The calculated numbers of neurons immunoreactive for P2X₂ receptor, calbindin, calretinin and choline acetyltransferase in the ileal submucosal plexus of the N, D and DR groups. ^aP < 0.05 vs N and DR groups. ChAT: Choline acetyltransferase.

Figure 7 Histograms showing the distribution of areas (μm²) of neurons immunoreactive for nitric oxide synthase (A), calbindin (B), calretinin (C) and choline acetyltransferase (D) in the ileal myenteric plexus of the N, D and DR groups. NOS: Nitric oxide synthase; ChAT: Choline acetyltransferase.

Figure 8 Histogram showing the distribution of areas (μm²) of neurons immunoreactive for calbindin (A), calretinin (B) and choline acetyltransferase (C) in the ileal submucosal plexus of the N, D and DR groups. ChAT: Choline acetyltransferase.