Published online Jun 15, 1998. doi: 10.3748/wjg.v4.i3.249
Revised: May 10, 1998
Accepted: May 20, 1998
Published online: June 15, 1998
AIM: To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera.
METHODS: Blood samples were collected from 278 patients with gastric diseases. A 3-day-old culture of H. pylori on chocolate blood agar was used to providespiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0.4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme-linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori.
RESULTS: Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non-ulcer dyspepsia (NUD) (44%) and other diseases (45%) (P < 0.05). IgG antibodies against spiral and coccoid antigens were detected in 50.7% (141/278) and 49.6% (138/278), respectively.
CONCLUSION: The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.