Guo BY, Zhang SY, Mukaida N, Harada A, Kuno K, Wang JB, Sun SH, Matshshima K. CCR5 gene expression in fulminant hepatitis and DTH in mice. World J Gastroenterol 1998; 4(1): 14-18 [PMID: 11819219 DOI: 10.3748/wjg.v4.i1.14]
Corresponding Author of This Article
Dr. Bao-Yu Guo, Department of Molecular Genetics, Second Military Medical University, 800 Xiangyi Road, Shanghai 200433, China
Article-Type of This Article
Original Articles
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Bao-Yu Guo, Shu-Han Sun, Department of Molecular Genetics, Second Military Medical University, Shanghai 200433, China
Su-Ying Zhang, Section of Scientific Research, Second Military Medical University, Shanghai 200433, China
Naofumi Mukaida, Akihisa Harada, Kouji Kuno, Jian-Bin Wang, Kouji Matshshima, Department of Pharmacology, Institute of Cancer Research, Kanazawa University, 13-1 Takara-machi, 920 Ishikawa, Japan
Bao-Yu Guo, male, born on 1953-06-30 in Xi’an, Shaanxi Province, graduated from the Second Military Medical University as a postgraduate in 1983, associate professor of molecular genetics, having 15 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Bao-Yu Guo, Department of Molecular Genetics, Second Military Medical University, 800 Xiangyi Road, Shanghai 200433, China
Received: August 21, 1997 Revised: September 25, 1997 Accepted: October 14, 1997 Published online: February 15, 1998
Abstract
AIMS: To isolate mouse CCR5 cDNA (muCCR5) and study its expression in vivo.
METHODS: Marathon PCR was used to isolate muCCR5 cDNA and two animal models were designed to investigate the gene expression in vivo, one was mouse fulminant hepatitis induced by Propionibacterium acnes (P.acnes) and the other was that with delayed type hypersensitivity reaction (DTH). A specific GST-NH2-terminus of muCCR5 fusion protein antibody F(ab’)2 was prepared and clarified. RT-PCR and immunohistochemical analysis were used to observe the expression level of CCR5 gene in mice.
RESULTS: A positive reaction of mouse macrophage was found in DTH but not expressed in P.acnes induced fulminant hepatitis by RT-PCR and immunohistochemical analysis.
CONCLUSION: This muCCR5 expression may be involved in an allergic processmediated by cellular immunity but not acute inflammatory reaction induced by P.acnes.
